Biological Sciences

Shuttle vector cloning process for yeast genomic library construction.

This illustration demonstrates the recombinant DNA cloning process, focusing on the use of a shuttle vector to create a yeast genomic library. The vector contains essential elements like the ampicillin resistance gene, URA3 for yeast selection, and multiple cloning sites for inserting DNA fragments. The process involves cutting the vector with BamHI, partially digesting yeast genomic DNA with Sau3A, and ligating the fragments into the vector. After transforming the recombinant plasmids into E. coli, successful transformations are screened for ampicillin resistance, and the plasmids are isolated.

This diagram is ideal for use in molecular biology courses, genetics research, scientific publications, and biotechnology education.

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